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Primer sequences are listed in S1 Table.

coli OP50 for 24 h in the dark.

Surprisingly, IF1-KO mice showed no evident phenotype and had no differences compared with wild-type animals in ATP synthesis or hydrolysis, F1Fo-ATPase dimer content or autophagy [17].

Animals that did not respond to touch were scored as dead.

Bunchu N, Vitta A, Thongwat D, Lamlertthon S, Pimolsri U, Waree P, Wongwigkarn J, Khamsri B, Cheewapat R, Wichai S.

elegans embryos and L1 larvae were mounted on agarose pads and cell corpses were quantify under Nomarski microscopy.

Informed consent form and a self-administered questionnaire were given to parents prior to pinworm screening.

The PCR products were gel-purified and fused by PCR overlap extension.

The supernatant was moved to a fresh tube and diluted 5-fold before measurement.

Total progeny included all the offspring that developed as larvae or died as embryos.